TOP >  研究紹介 >  細胞動態計測コア >  生体分子構造動態研究チーム


Takanori Kigawa



木川 隆則 (D. Sci.)

Kigawa Riken



  • NMR法を用いた細胞環境および細胞再現環境における生体分子の解析
  • 情報科学手法を活用したNMR測定・解析技術の開発
  • 無細胞タンパク質合成技術を用いた安定同位体標識技術の開発


  • Kigawa, T.
    "Advances in stable isotope assisted labeling strategies with information science"
    Arch. Biochem. Biophys., to be appeared, (2017)
  • Kasai, T., Nagata, K., Okada, M. & Kigawa, T.
    "NMR Spectral Analysis Using Prior Knowledge"
    J. Phys.: Conf. Ser. 699, 012003, (2016)
  • Okamura, H., Nishimura, H., Nagata, T., Kigawa, T., Watanabe, T. & Katahira, M.
    "Accurate and molecular-size-tolerant NMR quantitation of diverse components in solution"
    Sci. Rep. 6, 21742 (2016)
  • Kasai, T., Koshiba, S., Yokoyama, J. & Kigawa, T.
    "Stable isotope labeling strategy based on coding theory"
    J. Biomol. NMR 63, 213-221 (2015) doi:10.1007/s10858-015-9978-8
  • Numata, K., Motoda, Y., Watanabe, S., Osanai, T. & Kigawa, T.
    "Co-expression of Two Polyhydroxyalkanoate Synthase Subunits from Synechocystis sp PCC 6803 by Cell-Free Synthesis and Their Specific Activity for Polymerization of 3-Hydroxybutyryl-Coenzyme A"
    Biochemistry 54, 1401-1407 (2015) doi:10.1021/bi1501560h
  • Harada, R., Tochio, N., Kigawa, T., Sugita, Y. & Feig, M.
    "Reduced Native State Stability in Crowded Cellular Environment Due to Protein-Protein Interactions"
    J. Am. Chem. Soc. 135, 3696-3701 (2013) doi:10.1021/ja3126992
  • Matsuda, T., Watanabe, S. & Kigawa, T.
    "Cell-free synthesis system suitable for disulfide-containing proteins"
    Biochem. Biophys. Res. Commun. 431, 296-301 (2013) doi:10.1016/j.bbrc.2012.12.107
  • Matsuda, T., Furumoto, S., Higuchi, K., Yokoyama, J., Zhang, M., Yanai, K., et al.
    "Rapid biochemical synthesis of C-11-labeled single chain variable fragment antibody for immuno-PET by cell-free protein synthesis"
    Bioorganic & Medicinal Chemistry 20, 6579-6582 (2012) doi:10.1016/j.bmc.2012.09.038
  • Akama, S., Yamamura, M. & Kigawa, T.
    "A Multiphysics Model of In Vitro Transcription Coupling Enzymatic Reaction and Precipitation Formation"
    Biophys. J. 102, 221-230 (2012) doi:10.1016/j.bpj.2011.12.014
  • Yokoyama, J., Matsuda, T., Koshiba, S., Tochio, N. & Kigawa, T.
    "A practical method for cell-free protein synthesis to avoid stable isotope scrambling and dilution"
    Anal. Biochem. 411, 223-229 (2011) doi:10.1016/j.ab.2011.01.017