Unit Leader

Kenta Sumiyama (Ph.D.)

We are developing new techniques in mice genetic engineering including direct genome editing on fertilized eggs of mice and microdevice-based easy and precise aggregation method to obtain complete ES cell derived chimeric mice. By using these tools such as Triple CRISPR or Tol2 transgenesis, we are producing various useful genetic engineered mice within a short period of time.　We are also studying cis-regulatory mechanisms in non-coding region by using both molecular evolutionary analysis and genome editing technology to elucidate developmental and evolutionary mechanisms of mammals.

Research Topics

• Developing new techniques in highly efficient direct genome editing on fertilized eggs of mice
• Developing new transgenesis techniques on fertilized eggs of mice
• Study of cis-regulatory function in non-coding region of the mammalian genome
• Study of evolutionary mechanisms for acquiring novel function

Selected Publications

• Tatsuki, F., Sunagawa, G. A., Shi, S., Susaki, E. A., Yukinaga, H., Perrin, D., et al.
  "Involvement of Ca2+-Dependent Hyperpolarization in Sleep Duration in Mammals"
  Neuron 90, 70-85 (2016) doi:10.1016/j.neuron.2016.02.032
• Sunagawa, G.A., Sumiyama, K., Ukai-Tadenuma, M., Perrin, D. et al.
  "Mammalian Reverse Genetics without Crossing Reveals Nr3a as a Short-Sleeper Gene"
  Cell Reports 14, 662-677 (2016) doi:10.1016/j.celrep.2015.12.052
• Noguchi, M., Sumiyama, K. & Morimoto, M.
  "Directed Migration of Pulmonary Neuroendocrine Cells toward Airway Branches Organizes the Stereotypic Location of Neuroepithelial Bodies "
  Cell reports 13, 2679-2686 (2015) doi:10.1016/j.celrep.2015.11.058
• Goto, A., Nakahara, I., Yamaguchi, T., Kamioka, Y., Sumiyama, K., Matsuda, M., et al.
  "Circuit-dependent striatal PKA and ERK signaling underlies rapid behavioral shift in mating reaction of male mice "
  Proc. Natl. Acad. Sci. U.S.A. 112, 6718-6723 (2015) doi:10.1073/pnas.1507121112
• Amemiya, C. T., Alfoldi, J., Lee, A. P., Fan, S. H., Philippe, H., MacCallum, I., et al.
  "The African coelacanth genome provides insights into tetrapod evolution"
  Nature 496, 311-316 (2013) doi:10.1038/nature12027
• Sumiyama, K., Miyake, T., Grimwood, J., Stuart, A., Dickson, M., Schmutz, J., et al.
  "Theria-Specific Homeodomain and cis-Regulatory Element Evolution of the Dlx3-4 Bigene Cluster in 12 Different Mammalian Species"
  J. Exp. Zool. Part B-Mol. Dev. Evol 318, 639-650 (2012) doi:10.1002/jez.b.22469
• Kamioka, Y., Sumiyama, K., Mizuno, R., Sakai, Y., Hirata, E., Kiyokawa, E., et al.
  "Live Imaging of Protein Kinase Activities in Transgenic Mice Expressing FRET Biosensors"
  Cell Struct. Funct. 37, 65-73 (2012)
• Sumiyama, K., Kawakami, K. & Yagita, K.
  "A simple and highly efficient transgenesis method in mice with the Tol2 transposon system and cytoplasmic microinjection"
  Genomics 95, 306-311 (2010) doi:10.1016/j.ygeno.2010.02.006
• Sumiyama, K. & Ruddle, F. H.
  "Regulation of Dlx3 gene expression in visceral arches by evolutionarily conserved enhancer elements"
  Proc. Natl. Acad. Sci. U.S.A. 100, 4030-4034 (2003) doi:10.1073/pnas.0530119100
• Sumiyama, K., Irvine, S. Q., Stock, D. W., Weiss, K. M., Kawasaki, K., Shimizu, N., et al.
  "Genomic structure and functional control of the Dlx3-7 bigene cluster"
  Proc. Natl. Acad. Sci. U.S.A. 99, 780-785 (2002) doi:10.1073/pnas.012584999