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Laboratory for Nano-Bio Probes

Takashi Jin


Team Leader

Takashi Jin (D.Sci.)

Jin of RIKEN

Our research team develops novel nano-bio probes for visualization of molecular and cellular dynamics in biological systems. We will synthesize a variety of nano-bio-probes such as single-molecule imaging probes using quantum dots, cellular environment-sensitive probes, and cellular-state controllable probes using external magnetic fields. Furthermore, we will develop deep-tissue imaging probes to visualize cellular dynamics in vivo. We synthesize multi-colored fluorescent probes that emit NIR light (1000-1500 nm; the second optical window in living tissues), using cyanine dyes, fluorescent proteins, quantum dots, and rare-earth ions. By using the NIR fluorescent probes, cancer metastasis and immune reactions in vivo will be visualized at the cellular level. We are also developing probe delivery techniques using DDS for targeting cancer tissues and organs in small animals.

Research Topics

  • Multi-colored single-molecule imaging probes with high brightness and photo-stability
  • Cellular-environment sensitive near-infrared probes that respond to membrane potential, pH, and solvent polarity
  • Bioluminescence-coupled self illuminating near-infrared probes
  • Near-infrared probes that can be used in the second optical window (1000-1500 nm)
  • In vivo fluorescence imaging of cancer metastasis, immune/ inflammation responses at the cellular level
  • Probe delivery techniques for cells, tissues, and organs

Selected Publications

  • Tsuboi, S., Yamada, S., Nakane, Y., Sakata, T., Yasuda, H. & Jin, T.
    "Water-soluble near-infared fluorophores emitting over 1000 nm and their application to in vivo imaging in the second optical window (1000-1400nm)"
    ECS J. Solid State Sci. Technol. 7, R3093-3101 (2018)
  • Tsuboi, S., Sasaki, A., Sakata, T., Yasuda, H. & Jin, T.
    "Immunoglobulin binding (B1) domain mediated antibody conjugation to quantum dots for in vitro and in vivo molecular imaging"
    Chem. Commun. 53, 9450-9453 (2017) doi:10.1039/c7cc04966h
  • Jin, T., Tsuboi, S., Komatsuzaki, A., Imamura, Y., Muranaka, Y., Sakata, T., et al.
    "Enhancement of aqueous stability and fluorescence brightness of indocyanine green using small calix[4]arene micelles for near-infrared fluorescence imaging"
    Med. Chem. Commun 7, 623-631 (2016) doi:10.1039/c5md00580a
  • Komatsuzaki, A., Ohyanagi, T., Tsukasaki, Y., Miyanaga, Y., Ueda, M. & Jin, T.
    "Compact Halo-ligand-conjugated quantum dots for multicolored single-molecule imaging of overcrowding GPCR proteins on cell membranes"
    Small 11, 1396-1401 (2015) doi:10.1002/smll.201402508
  • Sasaki, A., Tsukasaki, Y., Komatsuzaki, A., Sakata, T., Yasuda, H. & Jin, T.
    "Recombinant protein (EGFP-Protein G)-coated PbS quantum dots for in vitro and in vivo dual fluorescence (visible and second-NIR) imaging of breast tumors"
    Nanoscale 7, 5115-5119 (2015) doi:10.1039/c4nr06480a
  • Tsukasaki, Y., Morimatsu, M., Nishimura, G., Sakata, T., Yasuda, H., Komatsuzaki, A., et al.
    "Synthesis and optical properties of emission-tunable PbS/CdS core-shell quantum dots for in vivo fluorescence imaging in the second near-infrared window"
    RSC Adv. 4, 41164-41171 (2014) doi:10.1039/c4ra06098a
  • Hasegawa, M., Tsukasaki, Y., Ohyanagi, T. & Jin, T.
    "Bioluminescence resonance energy transfer coupled near-infrared quantum dots using GST-tagged luciferase for in vivo imaging"
    Chem. Commun. 49, 228-230 (2013) doi:10.1039/c2cc36870f
  • Nakane, Y., Tsukasaki, Y., Sakata, T., Yasuda, H. & Jin, T.
    "Aqueous synthesis of glutathione-coated PbS quantum dots with tunable emission for non-invasive fluorescence imaging in the second near-infrared biological window (1000-1400 nm)"
    Chem. Commun. 49, 7584-7586 (2013) doi:10.1039/c3cc44000a
  • Ma, Q., Nakane, Y., Mori, Y., Hasegawa, M., Yoshioka, Y., Watanabe, T. M., et al.
    "Multilayered, core/shell nanoprobes based on magnetic ferric oxide particles and quantum dots for multimodality imaging of breast cancer tumors"
    Biomaterials 33, 8486-8494 (2012) doi:10.1016/j.biomaterials.2012.07.051