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研究業績 Publications


●  Michio Hiroshima and Masahiro Ueda (2024).
Automated single-molecule imaging for drug discovery
Proc. SPIE 12853 High-Speed Biomedical Imaging and Spectroscopy IX 1285308

●  Matsuoka, S, Iwamoto, K., Shin D.Y. and Ueda M. (2024).
Spontaneous Signal Generation by an Excitable System for Cell Migration
Frontiers in Cell and Developmental Biology 12: 1373609.(DOI:10.3389/fcell.2024.1373609)

●  Utsunomiya, S., Takebayashi, K., Yamaguchi, A., Sasamura, T., Inaki, M., Ueda, M., and Matsuno, K. (2024).
Left-right Myosin-Is, Myosin1C, and Myosin1D exhibit distinct single molecule behaviors on the plasma membrane of Drosophila macrophages
Genes to Cells, accepted. doi: 10.1111/gtc.13110

●  Takayama, M., Maeda, S., Watanabe, D., Takebayashi, K., Hiroshima, M. and Ueda M. (2024).
Cholesterol suppresses spontaneous activation of EGFR-mediated signal transduction
BBRC 704, 149673 (2024)



●  Banerjee T. Matsuoka S. Biswas D. Miao Y. Pal D. S. Kamimura Y. Ueda M. Devreotes P. N. and Iglesias P. A.(2023)
A dynamic partitioning mechanism polarizes membrane protein distribution
Nature Communications 14, Article number: 7909

●  Shin D. Y., Takagi H. Hiroshima M. Matsuoka S. and Ueda M. (2023)
Sphingomyelin metabolism underlies Ras excitability for efficient cell migration and chemotaxis
Cell Structure and Function 48: 145-160

●  Ouchida, T., Maeda, H., Akamatsu, Y., Maeda, M., Takamatsu, S., Kondo, J., Misaki, R., Kamada, Y., Ueda, M., Ueda, K. and Miyoshi, E. (2023)
The specific core fucose-binding lectin Pholiota squarrosa lectin (PhoSL) inhibits hepatitis B virus infection in vitro
Scientific Report 13, Article number: 6175 (2023)

●  廣島通夫, 上田昌宏 (2023)
生産と技術 vol.75, No.2, pp 77-81.

●  Takebayashi, K., Kamimura, Y. and Ueda M. (2023)
Field model for multistate lateral diffusion of various transmembrane proteins observed in living Dictyostelium cells
Journal of Cell Science 136 (4): jcs.260280.

●  特許出願
発明者:上田昌宏、廣島通夫、渡邉 大介
発明の名称: 1分子イメージングを用いた上皮成長因子受容体EGFRの活性評価に基づく薬剤スクリーニング法

●  上田 昌宏 (2023)
総論 生命システムにおけるゆらぎの階層性 - Organized Randomness in Biosystems –
特集 『揺らぎが奏でる生命機能』, 細胞 55(3), 2-3.

●  有賀 隆行 (2023)
特集 『揺らぎが奏でる生命機能』, 細胞 55(3), 8-11.

●  松岡 里実 (2023)
特集 『揺らぎが奏でる生命機能』, 細胞 55(3), 16-19.



●  Hashimura, H., Morimoto, Y. V., Hirayama, Y. and Ueda, M.(2022)
Calcium responses to external mechanical stimuli in the multicellular stage of Dictyostelium discoideum
Scientific Report 12, Article number: 12428.

●  Kawase, N., Sugihara, A., Kajiwara, K., Hiroshima, M., Akamatsu, K., Nada, S., Matsumoto, K., Ueda, M. and Okada M. (2022).
SRC kinase activator CDCP1 promotes hepatocyte growth factor-induced cellmigration/invasion of a subset of breast cancer cells
J. Biol. Chem. 298, 101630.(DOI: 10.1016/j.jbc.2022.101630)

●  Kawakami, K., Yanagawa, M., Hiratsuka, S., Yoshida, M., Ono, Y., Hiroshima, M., Ueda, M., Aoki, J., Sako, Y. and Inoue A. (2022).
Heterotrimeric Gq act as a switch for GRK5/6 selectivity underlying β-arrestin transducer bias
Nature Communications 13, Article number: 487.(DOI: 10.1038/s41467-022-28056-7)



●  Kuwashima, Y., Yanagawa, M., Abe, M., Hiroshima, M., Ueda, M., Arita M. and Sako Y. (2021)
Comparative analysis of single-molecule dynamics of TRPV1 and TRPV4 channels in living cells In Applications of Advanced Imaging and Analytical Microscopic Techniques on Molecular and Cell Biology
International Journal of Molecular Sciences 22(16), 8473.

●  Ohtsuka, D., Ota, N., Amaya, S., Matsuoka, S., Tanaka, Y., and Ueda, M. (2021)
A sub-population of Dictyostelium discoideum cells shows extremely high sensitivity to cAMP for directional migration
Biochem. Biophys. Res. Commun., 554: 131-137(doi: 10.1016/j.bbrc.2021.03.095)

●  Kamimura,Y., and Ueda, M. (2021)
Different heterotrimeric G protein dynamics for wide-range chemotaxis in eukaryotic cells In Dictyostelium: A Tractable Cell and Developmental Model in Biomedical Research.
(eds. Robin Williams and Robert Huber and Annette Müller)
Frontiers in Cell and Developmental Biology, 9: 724797.

●  Kamimura,Y., and Ueda, M. (2021)
GPCR signaling regulation in Dictyostelium chemotaxis
Methods in Molecular Biology, 2274: 317-336.

●  松岡里実、上田昌宏 (2021)
特集 『生物物理学の進歩 – 生命現象の定量的理解に向けて』
生体の科学 72巻3号, pp. 229-233.



●  Yoshioka, D., Fukushima, S., Koteishi, H., Okuno, D., Ide, T., Matsuoka, S., and Ueda, M. (2020)
Single-molecule imaging of PI(4,5)P2 and PTEN in vitro reveals a positive feedback mechanism for PTEN membrane binding
Communications Biology 3, Article number: 92. (doi: 10.1038/s42003-020-0818-3)

●  Yamazaki, S., Hashimura, H., Morimoto, Y. V., Miyanaga, Y., Matsuoka, S., Kamimuraa,Y., and Ueda, M. (2020)
Talin B regulates collective cell migration via PI3K signaling in the mound of Dictyostelium discoideum
Biochem. Biophys. Res. Commun., accepted. (doi: 10.1016/j.bbrc.2020.02.060.)

●  Hiroshima, M., Yasui, M., and Ueda, M. (2020)
Large scale single-molecule imaging aided by artificial intelligence
Microscopy, dfz116. (doi: 10.1093/jmicro/dfz116 )

● 上村陽一郎、上田昌宏(2020)
“三量体G蛋白質シャトリング制御 ” 医学のあゆみ, Volume 272, Issue 7, pp 605-606



●  Fukushima, S., Matsuoka, S. and Ueda, M. (2019)
Excitable dynamics of Ras triggers spontaneous symmetry breaking of PIP3 signaling in motile cells
Journal of Cell Science 132(5), pii: jcs224121. (doi: 10.1242/jcs.224121)

●  Hashimura, H., Morimoto, Y. V., Yasui, M. and Ueda, M. (2019)
Collective cell migration of Dictyostelium without cAMP oscillations at multicellular stages
Communications Biology 2, Article number: 34. (doi: 10.1038/s42003-018-0273-6)



●  Tanabe, Y., Kamimura, Y., and Ueda, M. (2018)
Parallel signaling pathways regulate excitable dynamics differently for pseudopod formation in eukaryotic chemotaxis
Journal of Cell Science, 131: jcs214775. (doi: 10.1242/jcs.214775)

●  Miyanaga, Y., Kamimura, Y., Kuwayama, H., Devreotes, P.N., and Ueda, M. (2018)
Chemoattractant receptors activate, recruit and capture G proteins for wide range chemotaxis
Biochem. Biophys. Res. Commun., 507: 304-310. (doi: 10.1016/j.bbrc.2018.11.029)

●  Miyagawa, T., Koteishi, H., Kamimura, Y., Miyanaga, Y., Takeshita, K., Nakagawa, A., and Ueda, M. (2018)
Structural basis of Gip1 for cytosolic sequestration of G-protein in wide range chemotaxis
Nature Communications 9, Article number: 4635 (doi: 10.1038/s41467-018-07035-x)
プレスリリースは こちら

●  Matsuoka, S., and Ueda, M. (2018).
Mutual inhibition between PTEN and PIP3 generates bistability for polarity in motile cells
Nature Communications 9, Article number: 4481 (doi: 10.1038/s41467-018-06856-0)
理研のプレスリリースは こちら

●  Yasui M., Hiroshima M., Kozuka J., Sako Y., and Ueda M. (2018)
Automated single-molecule imaging in living cells
Nature Communications, 9, Article number: 3061 (doi: 10.1038/s41467-018-05524-7)
理研のプレスリリースは こちら

●  Yanagawa, M., Hiroshima, M., Togashi, Y., Abe, M., Yamashita, T., Shichida ,Y., Murata, M., Ueda, M. and Sako, Y. (2018)
Single-molecule diffusion-based estimation of ligand effects on G protein-coupled receptors
Science Signaling, Vol. 11, Issue 548, eaao1917. (doi: 10.1126/scisignal.aao1917)
理研のプレスリリースは こちら

●  Pervin, M.S., Itoh, G., Talukder, M.S.U., Fujimoto, K., Morimoto, Y.V., Tanaka, M., Ueda, M., Yumura, S. (2018)
A study of wound repair in Dictyostelium cells by using novel laserporation
Scientific Report, 8: 7969. (doi: 10.1038/s41598-018-26337-0.)

●  Hiroshima, M., Pack, C.G., Kaizu, K., Takahashi, K., Ueda, M., and Sako, Y. (2018)
Transient acceleration of epidermal growth factor receptor dynamics produces higherorder signaling clusters
Journal of Molecular Biology, 430: 1381-1396. (DOI: 10.1016/j.jmb.2018.02.018.)

● 宮永之寛、上村陽一郎、上田昌宏 (2018)
“三量体Gタンパク質の局在制御を介した走化性レンジの拡張機構” 生物物理 58 巻 5 号, p. 237-240. (doi 10.2142/biophys.58.237)

● 廣島通夫、上田昌宏 (2018)
“超解像顕微鏡, PALM/STORM” 『生きてるものは全部観る!イメージングの選び方・使い方100+』 実験医学, 36巻, p. 236



●  Sato, R., Kozuka, J., Ueda, M., Mishima, R., Kumagai, Y., Yoshimura, A., Minoshima, M., Mizukami, S., and Kikuchi, K. (2017)
Intracellular protein-labeling probes for multicolor single-molecule imaging of immune receptor-adaptor molecular dynamics
Journal of the American Chemical Society, in press. (DOI: 10.1021/jacs.7b08262)



●  Matsuoka, S., Miyanaga, Y. and Ueda, M. (2016)
Multi-state Transition Kinetics of Intracellular Signaling Molecules by Single-Molecule Imaging Analysis
Methods in Molecular Biology, 1407:361-79. (doi: 10.1007/978-1-4939-3480-5_25)

●  Kamimura, Y., Miyanaga, Y., and Ueda, M. (2016)
Heterotrimeric G protein shuttling via Gip1 extends the dynamic range of eukaryotic chemotaxis
Proc. Natl. Acad. Sci. USA., 113 (16): 4356–4361. (doi: 10.1073/pnas.1516767113)



●  Komatsuzaki, A., Ohyanagi, T., Tsukasaki, Y., Miyanaga, Y., Ueda, M., and Jin, T. (2015)
Compact Halo-Ligand Conjugated Quantum Dots for Multicolored Single-Molecule Imaging of Overcrowding GPCR Proteins on Cell Membrane
Small 11(12): 1396-1401 (doi: 10.1002/smll.201402508)

●  Watabe, M., Arjunan, S.N.V., Fukushima, S., Iwamoto, K., Kozuka, J., Matsuoka, S., Shindo, Y., Ueda, M. and Takahashi, K. (2015)
A computational framework for bioimaging simulation.
PLoS One, 10(7): e0130089. (doi: 10.1371/journal.pone.0130089)

●  Ishibashi, M., Miyanaga, Y., Matsuoka, S., Kozuka, J., Togashi, Y., Kinashi, T. and Ueda, M. (2015).
Integrin LFA-1 regulates cell adhesion via transient clutch formation
Biochem. Biophys. Res. Commun., 464 (2):459-466. (doi:10.1016/j.bbrc.2015.06.155)

● 松岡里実、上田昌宏 (2015)
“イノシトールリン脂質シグナル” 生体の科学, 66巻5号, 増大特集 『細胞シグナル操作法』 pp392-393.



●  Komatsuzaki, A., Ohyanagi, T., Tsukasaki, Y., Miyanaga, Y., Ueda, M., and Jin, T. (2014)
Compact Halo-Ligand Conjugated Quantum Dots for Multicolored Single-Molecule Imaging of Overcrowding GPCR Proteins on Cell Membrane
Small. article first published online: 12 DEC 2014. (doi: 10.1002/smll.201402508)

●  Yasui, M., Matsuoka, S., and Ueda M. (2014)
PTEN hopping on the cell membrane is regulated via a positively-charged C2 domain.
PLOS Computational Biology. 10, e1003817. (doi: 10.1371/journal.pcbi.1003817)

●  Shinohara, H., Behar, M., Inoue, K., Hiroshima, M., Yasuda, T., Nagashima, T., Kimura, S., Sanjo, H., Maeda, S., Yumoto, N., Ki, S., Akira, S., Sako, Y., Hoffmann, A., Kurosaki, T., and Okada-Hatakeyama M. (2014)
Positive Feedback Within a Kinase Signaling Complex Functions as a Switch Mechanism for NF-κB Activation.
Science 344: 760-764 (doi: 10.1126/science.1250020).

●  Cai, H., Katoh-Kurasawa, M., Muramoto, T., Santhanam, B., Long, Y., Li, L., Ueda, M., Iglesias, P. A., Shaulsky, G., and Devreotes, P, N. (2014) .
Nucleocytoplasmic shuttling of a GATA transcription factor functions as a development timer.
Science 343(6177):1249531. (doi: 10.1126/science.1249531)

●  Morimoto, YV., Ito, M., Hiraoka, KD., Che, YS., Bai, F., Kami-Ike, N., Namba, K., and Minamino, T.(2014).
Assembly and stoichiometry of FliF and FlhA in Salmonella flagellar basal body.
Molecular Microbiology (doi:10.1111/mmi.12529)

●  Morimoto, YV., and Minamino, T.(2014).
Structure and function of the bi-directional bacterial flagellar motor.
Biomolecules 4: 217-234 (doi:10.3390/biom4010217)

●  Masatoshi Nishikawa, Marcel Hörning, Masahiro Ueda and Tatsuo Shibata (2014).
Excitable signal transduction induces both spontaneous and directional cell asymmetries in the Phosphatidylinositol Lipid Signaling System for Eukaryotic Chemotaxis.
Biophysical Journal, 106, 723-734. (doi:10.1016/j.bpj.2013.12.023)

● 高木拓明,佐藤雅之,上田昌宏 (2014)
“細胞運動のゆらぎから探るロバストな細胞情報処理機構” ,細胞工学33巻1号(2014年1月号),pp48-54.

● 上田昌宏 (2014)

● 柴田達夫,西川正俊,上田昌宏 (2014)
細胞工学 33巻6号(2014年6月号),pp592-597

● 松岡里実,上田昌宏 (2014)
生体の科学, 増大特集 『生命動態システム科学』, 65巻5号 pp394-395

● 松岡里実,上田昌宏 (2014)
"細胞表面における情報処理" 『1分子生物学』(化学同人), pp. 139-147.

● 佐甲靖志、廣島通夫、日比野佳代 (2014)
"細胞内情報処理反応の1分子計測:タンパク質ダイナミクスと分子認識"『1分子ナノバイオ計測』(化学同人), pp. 180–189.

● 日比野佳代 (2014)
"1分子FRET計測"  『1分子生物学』(化学同人), pp. 205–213.



●  Kuwayama, H., Miyanaga, Y., Urushihara, H. and Ueda, M. (2013).
A RabGAP Regulates Life-Cycle Duration via Trimeric G-protein Cascades in Dictyostelium discoideum.
PLOS ONE, 8, e81811. (doi:10.1371/journal.pone.0081811)

●  Shibata, T., Nishikawa, M., Matsuoka, S., and Ueda, M. (2013).
Intracellular encoding of spatiotemporal guidance cues in a self-organizing signaling system for chemotaxis in Dictyostelium cells.
Biophys. J., 105:, 2199-2209. (doi:10.1016/j.bpj.2013.09.024)

● Okamoto, M., Namba, T., Shinoda, T., Kondo, T., Watanabe, T., Inoue, Y., Takeuchi, K., Enomoto, Y., Ota, K., Oda, K., Wada, Y., Sagou, K., Saito, K., Sakakibara, A., Kawaguchi, A., Nakajima, K., Adachi, T. Fujimori, T., Ueda, M., Hayashi, S., Kaibuchi, K. and Miyata, T. (2013).
TAG-1-assisted progenitor elongation streamlines nuclear migration to optimize subapical crowding.
Nature Neuroscience, (doi:10.1038/nn.3525)

● Hibino, K., Hiroshima, M., Nakamura Y., and Sako Y. (2013).
Single-Molecule Imaging Measurements of Protein-Protein Interactions in Living Cells.
Living Cells. InTech Chapter 17, (doi:10.5772/52386)

● Masud Rana, A.Y. K. Md., Tsujioka, M., Miyagishima, S., Ueda, M. and Yumura, S. (2013).
Dynamin contributes to cytokinesis by stabilizing actin filaments in the contractile ring.
Genes to Cells, 18, 621-635. (doi:10.1111/gtc.12060)

● Matsuoka, S.‚ Shibata, T.‚ and Ueda‚ M. (2013).
Asymmetric PTEN distribution regulated by spatial heterogeneity in membrane-binding state transitions.
PLoS Computational Biology. 9, e1002862. (doi:10.1371/journal.pcbi.1002862)

● Morimoto, Y.V.‚ Nakamura, S.‚ Hiraoka, K.D.‚ Namba, K.‚ and Minamino, T. (2013).
Distinct roles of highly conserved charged residues at the MotA-FliG interface in bacterial flagellar motor rotation.
J. Bacteriol. 195, 474-481.

● 上田昌宏 (2013)
“真核細胞の走化性の物理”, パリティー(丸善)28巻12号(2013年12月号),pp24-32.

● 廣島道夫,佐甲靖志 (2013)
“1分子キネティクス解析により示されたErbB受容体の反応調節機構”, 生物物理53巻6号,pp317-318.



● Lee, J.‚ Miyanaga, Y.‚ Ueda, M. and Hohng, S. (2012).
Video-rate confocal microscopy for single-molecule imaging in live cells and super-resolution fluorescence imaging.
Biophys. J. 103: 1691-1697. (doi:10.1016/j.bpj.2012.09.014)

● Hara, N.‚ Morimoto, Y.V.‚ Kawamoto, A.‚ Namba, K. and Minamino, T. (2012).
Interaction of the extreme N-terminal region of FliH with FlhA is required for efficient bacterial flagellar protein export.
J. Bacteriol. 194, 5353-5360.

● Shibata, T.‚ Nishikawa‚ M.‚ Matsuoka‚ S. and Ueda‚ M. (2012).
Modeling the self-organized phosphatidylinositol lipids signaling system in chemotactic cells based on quantitative image analysis.
Journal of Cell Science. 125, 5138–5150. (doi:10.1242/jcs.108373)

● Tsujioka‚ M. Yumura‚ S.‚ Inouye, K.‚ Patel‚ H.‚ Ueda‚ M. and Yonemura‚ S. (2012).
Talin couples the actomyosin cortex to the plasma membrane during rear retraction and cytokinesis.
Proc. Natl. Acad. Sci. USA. 109, 12992-12997. (doi:10.1073/pnas.1208296109)

● Nishimura‚ S. I.‚ Ueda‚ M. and Sasai‚ M. (2012).
Non-Brownian dynamics and strategy of amoeboid cell locomotion.
Physical Review E. 85, 041909.



● Matsuoka‚ S. (2011).
Statistical Analysis of Lateral Diffusion and Reaction Kinetics of Single Molecules on Membranes of Living Cells.
In Cell Signaling Reactions: Single-Molecule Kinetic Analysis. (Sako‚ Y. and Ueda‚ M. eds).
Springer-Verlag. 265-296

● Miyanaga‚ Y. and Ueda, M. (2011).
Single-Molecule Kinetic Analysis of Stochastic Signal Transduction Mediated by G-protein Coupled Chemoattractant Receptors.
In Cell Signaling Reactions: Single-Molecule Kinetic Analysis. (Sako‚ Y. and Ueda‚ M. eds).
Springer-Verlag. 33-57

● Tsujioka, M. (2011).
Cell migration in multicellular environments.
Develop. Growth Differ. 53(4), 528-537.

● 柴田達夫,上田昌宏
「生命科学の新しい潮流 理論生物学」 共立出版 編集 望月敦史、p.97-115.

● 松岡里実,上田昌宏
“確率的な細胞内シグナル処理の1分子イメージング解析”‚ 最新医学 第66巻,第10号‚pp.100-106 (2011).