I'm now challenging to clarify the mechanism of unique biological function (adaptability, robustness, flexibility, low-energy cost etc.) at biomolecular assembly level.
One of my key technologys is DNAorigami and I'm collaborationg with Prof. William Shih at Harvard University. The technology enables us to spatially control the positioning of bio molecules in a nano- to meso-scale space by utilizing the ability of programable self-assembly of DNA. By combining single molecule measurement techniques and DNAorigmai, I'm exploring the internal dynamics of biomolecular assembly system and the system behaviour itself.
I named my goal "Nanobio robotics". but unlike artificial robot, I expect unique system composed of Brownian type nanomachines that have been revealed by our previous research. I aim at understanding design principle of current biological system and as a spin-off, constructing novel system directed to the field of medical and engineering applications.
Brownian machine cannnot ignore random fluctuation and the output also fluctuates even in the same input signal depending on the internal state of the system. Fumio Oosawa proposed the concept as "Loose-coupling". Such elements feels to be negative and unreliable property from a engineering point of view, but may be a positive one to realize stable and reliable system agains various perturbation. I would like to understand the hypothesis by a synthetic approach.
I'm now pushing on themes below
1-1 Design of motor protein assembly as a Loose coupling system
1-2 Measurement of internal dynamics of motor protein assembly
1-3 Methodology for simultaneous measurement of the internal
dynamics and sytem behaviour
2. Design of single molecule measurement system of central dogma
3. Development of DNA origami tool for the in vitro, in vivo single